四川大学学报（自然科学版）2012，Vol.49Issue(2)：436-440,5.DOI:10.3969/j.issn.0490-6756.2012.02.033
食道癌细胞在异鼠李素处理中应激作用的研究
The study on cellular stress response in isorhamnetin-treated Eca-109 cells
摘要
Abstract
To explore the molecular mechanisms of cellular stress response in the isorhamnetin-treated Eca-109 cells on the basis of the previous research in our lab. Data showed that the proliferation of Eca-109 cells was not absolutely arrested initially but still continued proliferation within the 72 h when these cells were exposed to isorhamnetin. It was found that the expression levels of NF-kB/p65 and NF-kB/ p50 were increased in nuclei at first. The expressions of COX-2, bcl-2 and mcl-1 were up-regulated with the treatment of isorhamnetin at first. IκBa and Bax were down-regulated initially but then up-regulated. After treated with MG-132 additionally, the nuclei expressions of p50/p65 were decreased and the proliferation of isorhamnetin-treated Eca-109 cells within the initial 72 h was inhibited. In conclusion, during the initial exposure to isorhamnetin, cellular stress response activated NF-kB signaling pathway, then changed the expression levels of the downstream gene expression to inhibit the apoptosis induced by isorhamnetin in the initial 72 h. But then the apoptosis induced by isorhamnetin plays a dominant role, which inhibits the NF-kB pathway and the proliferation of Eca-109 cells.关键词
异鼠李素/Eca-109/NF-κB/细胞应激Key words
sorhamnetin,Eca-109,NF-kB,cellular stress分类
医药卫生引用本文复制引用
杨超,蔡哲,石超,范林洋,杨春蕾..食道癌细胞在异鼠李素处理中应激作用的研究[J].四川大学学报（自然科学版）,2012,49(2):436-440,5.基金项目
四川省科技厅项目(2007SGY013) （2007SGY013）
