四川大学学报（自然科学版）2012，Vol.49Issue(2)：459-464,6.DOI:10.3969/j.issn.0490-6756.2012.02.037
抗β8整合素单克隆抗体在推断小鼠死亡时间中的初步应用
Application of anti-β8 integrin monoclonal antibody in determination of postmortem interval in mice
摘要
Abstract
Anti-β8 integrin monoclonal antibody was employed to detect the expression and location of β8 integrin on astrocytes in a oxygen-glucose deprivation (OGD)-reoxygenation model> as well as in kidney and brain in the different time points after postmortem in mice, then a density (mean)- postmortem interval curve was drawn to speculate the postmortem interval of mice. It was showed: the expression of β8 integrin on astrocytes, tubular epithelial cells and glial cells was increased in hypoxia. The location of β8 integrin transferred from cytomembrane to cytoplasm,and finally β8 integrin was released to extracellular matrix along with fracture of cytomembrane. Image-Pro Plus analysis indicated that β8 integrin expression of glial cells peaked at 48 h in hypoxia. The curve of density (mean)- postmortem interval was able to speculate the postmortem interval accurately.关键词
β8整合素/单克隆抗体/死亡时间/Image-Pro PlusKey words
β8 integrin,monoclonal antibody,postmortem interval,Image-Pro Plus分类
医药卫生引用本文复制引用
黎光,张晨阳,魏大鹏,章崇杰,刘彦军,胡丽娟,罗志娟..抗β8整合素单克隆抗体在推断小鼠死亡时间中的初步应用[J].四川大学学报（自然科学版）,2012,49(2):459-464,6.基金项目
四川大学青年教师科研启动基金(2009SCU11149) （2009SCU11149）
