四川大学学报（自然科学版）2012，Vol.49Issue(2)：465-470,6.DOI:10.3969/j.issn.0490-6756.2012.02.038
重组风雨花凝集素在大肠杆菌中表达条件的优化
Optimization of recombinant Zephyranthes grandiflora Agglutinin expression in Escherichia coli
摘要
Abstract
In order to inerease the expression of recombinant Zephyranthes grandiflora Agglutinin (ZGA), the influences of culture conditions such as pH, temperature, inducting time, IPTG concentration and carbon sources in growth medium, volume of medium, inoculum quantities, inoculum time and concentration of inorganic ions, I. E. Mg2+, Ca2+ , NH4+ et al on expression of recombinant protein were studied. The results after optimization showed that the expression of recombinant fusion ZGA was 61. 8% of the total bacteria protein, increasing about 13. 4%. The selected clone was amplified in 2 Liter LB culture medium and induced by IPTG, and 6 g/L thalli per liter culture medium were obtained under optimized conditions. The thalli was selected and homogenized under high pressure. SDS-PAGE demonstrated that the recombinant targeted proteins were mainly indwelled in the inclusion body.关键词
风雨花凝集素/大肠杆菌/表达条件/重组蛋白/包涵体Key words
Zephyranthes grandi flora Agglutinin (ZGA), E.coli, expression condition, recombinant protein, inclusion bodies分类
生物科学引用本文复制引用
张帆,高顺,温馨,谢之易,鲍锦库..重组风雨花凝集素在大肠杆菌中表达条件的优化[J].四川大学学报（自然科学版）,2012,49(2):465-470,6.基金项目
国家自然科学基金(30670469 （）
30970643) （）
