水产学报2012，Vol.36Issue(3)：350-358,9.DOI:10.3724/SP.J.1231.2012.27748
斑点叉尾(鮰)源海豚链球菌DGX07株simA基因克隆、鉴定及分子特性分析
Cloning,identification and molecular characteristics analysis of simA gene of a Streptococcus iniae strain DGX07 from channel catfish
摘要
Abstract
The RAPD analysis of Streptococcus iniae virulent strain DGX07 isolated from channel catfish (Ictalurus punctatus)v/as conducted by PCR with Primer pl4(5'-GATCAAGTCC-3'). The simA gene of DGX07 was amplified by PCR with specific primers and cloned into pMD19-T vector. The positive plasmid was selected and identified by PCR and digestion of double restriction enzyme (BamH I + Xho I ). Molecular characterization analysis of simA gene was performed by bioinformatics tools. The results showed that S. Iniae simA amino acid sequence was highly conservative and with 100% homology to the simA and simB of S. Iniae isolated. The polypeptide contained a signal peptide comprising 41 amino acids and Bap31 and Gram _ pos _ anchor functional domains. The polypeptide had some important sites related to post-translational modification, including 22 phosphorylation sites and 2 N-glycosylation sites. The hydrophilic regions were larger than hydrophobic regions in polypeptide chain. The 5. Iniae simA was extramembranous protein with multiple antigen advantaged regions. The analysis of codon bias indicated the codon usage frequency of 5. Iniae simA different,and the codon bias of S. Iniae simA preferred the yeasts. Gene accession number :JF330100.关键词
斑点叉尾(鮰)/海豚链球菌/simA基因/克隆Key words
Ictalurus punctatus/ Streptococcus iniae/ simA gene/ clone分类
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汪开毓,王均,肖丹,陈德芳,黄锦炉,黄凌远,付希,王浩丞..斑点叉尾(鮰)源海豚链球菌DGX07株simA基因克隆、鉴定及分子特性分析[J].水产学报,2012,36(3):350-358,9.基金项目
教育部创新团队项目(IRT0848) （IRT0848）
通威股份有限公司重点资助项目(2006-2009) （2006-2009）
