四川中医2012，Vol.30Issue(4)：14-16,3.
消癌解毒方对肝癌细胞株H22凋亡及Survivin表达的影响
The Effect of Xiaoai Jiedu Recipe (XJR) on Apoptosis and Survivin Expression in Hepatocarcinoma Cell Line of H22
摘要
Abstract
Objective: To investigate the effect of Xiaoai Jiedu Recipe (XJR) on apoptosis and Survivin expression in hep-atocarcinoma cell line of H22, and to study the possible anti-tumor mechanisms. Methods; Establishing the mice transplanted H22 hepatocarcinoma tumor model, control group, XJR sub-group of low, medium and high dose (10, 30, 90mg/kg) group and cisplatin (0. 00lmg/kg) treatment group were devided, we used flow cytometry technique to detect the tumor cell cycle and apoptosis in each group, Western blot assay tested the expression of Survivin protein of transplanted hepatocarcinoma H22 tumor cells. Results; The average apoptosis rate of the mice in each dose group of Xiaoai Jiedu Recipe (XJR) and cisplatin positive control group was higher than the model group mice. Comparing the two groups, the differences were statistically significant ( P <0. 01, P<0. 05). FCM cell cycle showed that compared with model group, the proportion of cells in G0/G1 period of XJR medium dose group and cisplatin positive control group increased relatively, P<0. 05, the difference was statistically significant. By Western blot analysis, compared with model group, the expression of Survivin protein in each group were decreased, and DDP positive control group decreased obviously, followed by XJP sub-group of low, high and medium dose (30, 10, 90mg/ kg) group, respectively. Conclusion; Xiaoai Jiedu Recipe (XJR) can promote tumor cell apoptosis and inhibit expression of Survivin protein, which is one of the mechanisms of its anti-tumor effects.关键词
消癌解毒方/H22/肝癌细胞/移植瘤/Surviving/细胞凋亡Key words
Xiaoai Jiedu Recipe (XJR) /H22 hepatoma cells/Transplanted hepatocarcinoma/Survivin /Apoptosis分类
医药卫生引用本文复制引用
卢伟,沈波,李黎,陈海彬,吴勉华..消癌解毒方对肝癌细胞株H22凋亡及Survivin表达的影响[J].四川中医,2012,30(4):14-16,3.基金项目
国家自然科学基金资助项目(No,30973742/C190201) （No,30973742/C190201）
江苏省自然科学基金资助项目(No.BK2007240) （No.BK2007240）
江苏省高校自然科学研究面上项目资助(No.09KJB360003):江苏省普通高校研究生科研创新项目资助(No.CX09S-037Z) （No.09KJB360003）
江苏省中医药局课题(No.LB09032) （No.LB09032）
