首都师范大学学报（自然科学版）2012，Vol.33Issue(1)：39-44,6.
嗜热菌铁硫簇合成途径中SufB、SufC和SufD蛋白的表达、纯化及其功能初步研究
Expression, Purification and Preliminary Function Analysis of sufB sufC sufD from Thermus thermophilic HB8
王妮莎 1张宗棋 1刘晓晴1
作者信息
- 1. 首都师范大学生命科学学院,北京100048
- 折叠
摘要
Abstract
Iron-sulfur cluster,ubiquitous in all living organisms, is one of the oldest living matter. In this paper, sufB, sufC,sufD were cloned by PCR using HB8 genome as a template. Three protein expression vectors, su/Z?-pET28a ( + ) , sufC-pET28a ( + ) , sjf/D-pGEX-6P-l were constructed and transferred into Rosetta ( DE3 ) for protein expression. Proteins of Thermus thermophilic HB8 have thermal stability, which is conducive to reconstructing the complex and some preliminary functional studies. Further research on the mechanism is continued and processing.关键词
铁硫簇/SufC/融合表达/亲和层析/荧光共振能量转移Key words
Fe-S scaffold/ SufC / Fusion expression/ affinity chromatogrphy/ FRET分类
生物科学引用本文复制引用
王妮莎,张宗棋,刘晓晴..嗜热菌铁硫簇合成途径中SufB、SufC和SufD蛋白的表达、纯化及其功能初步研究[J].首都师范大学学报（自然科学版）,2012,33(1):39-44,6.
