首都师范大学学报(自然科学版)2012,Vol.33Issue(2):47-52,6.
微型DNA条形码在鱼类识别上的应用
Mini DNA Barcode Utilizes in Fish Classification
摘要
Abstract
We got 53 COI sequences of fish from Huairou area of Beijing. Combined with classification, the data from minimalist DNA barcode and DNA barcode were compared to testify if the minimalist DNA barcode was available for species identification. We divided 53 COI sequences into 3 segments, calculated the accuracy of these segments by carrying out neighbor-joining ( NJ) analysis and analyzing the genetic distance. The results indicated that molecular identification consistent with the morphological identification is probably, and found two errors in classification. The accuracy of the first 200 bp barcode in species identification was consistent with the result of full COI. So it can be concluded that the identification of fish species could be done by minimalist DNA barcode. It will benefit the development and protection of fish.关键词
鱼类分类/生物入侵/DNA条形码/DNA微型条码Key words
fish classification/ biological invasion/ DNA barcode/ mini barcode分类
生物科学引用本文复制引用
程鹏,张爱兵,王忠锁..微型DNA条形码在鱼类识别上的应用[J].首都师范大学学报(自然科学版),2012,33(2):47-52,6.基金项目
国家自然基金资助(Nr.31071963) (Nr.31071963)