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人工培养北虫草高效液相色谱指纹图谱研究

金田 宋健 李洁 石俊英

山东中医药大学学报2012,Vol.36Issue(2):153-155,3.
山东中医药大学学报2012,Vol.36Issue(2):153-155,3.

人工培养北虫草高效液相色谱指纹图谱研究

Study on HPLC fingerprint spectrum of cultured Cordyceps Militaris

金田 1宋健 2李洁 2石俊英2

作者信息

  • 1. 山东中医药大学,山东济南250355
  • 2. 山东中医药大学药学院,山东济南250355
  • 折叠

摘要

Abstract

Objective:To establish the HPLC fingerprint spectrum of cultured Cordyceps mililaris in different areas. Methods;Use methanol and water as mobile phase,gradient elution by HPLC. The detection wavelength was 260 nm, the similarity of the spectrum was analyzed. Results:There were 10 common peaks in the fingerprint spectrum. The similarities between every spectrum and the common one are more than 0.9. The result of methodology determination were fitted to the request. Conclusion:This method is ease to handle,credible and has a good repeatability as an effective measure to control the quality of cultured Cordyceps mililaris.

关键词

人工培养北虫草/高效液相色谱/指纹图谱

Key words

cultured Cordyceps mulitaris/ HPLC/ fingerprint spectrum

分类

医药卫生

引用本文复制引用

金田,宋健,李洁,石俊英..人工培养北虫草高效液相色谱指纹图谱研究[J].山东中医药大学学报,2012,36(2):153-155,3.

基金项目

山东省科技发展计划项目(编号:2010GSF10277) (编号:2010GSF10277)

山东中医药大学学报

OACSTPCD

1007-659X

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