山东农业科学2012，Vol.44Issue(3)：17-20,4.
苹果叶片愈伤组织的诱导培养
Induction Culture of Callus from Apple Leaves
摘要
Abstract
In order to obtain better callus, the leaves of Rails and Fuji apple were used as explants, and the effects of different disinfection methods, types and concentrations of plant growth regulators, light conditions and ways of explants touching medium on callus induction were studied. The results were as follows. (1) The callus induction rate of only light culture after inoculation was only 50% ~55%. But after dark culture for 14 or 21 days and then light culture, the callus induction rate reached to 100% , and yellow calluses with tight structure were obtained from the former, while albino ones with loose structure were obtained from the latter. Dark culture for 14 days was advantage to callus formation. (2) The MS basic medium with no hormone was beneficial to reducing the browning rate compared to the MS medium with added hormones. (3) The induction rate could increased by 25% -30% and the callus formation time was earlier with the leaf abaxial surface contacting medium compared to using the leaf adaxial surface contacting medium. (4) The suitable medium for callus formation of Rails and Fuji leaves were MS + 2.0 mg/L 6 - BA + 0. 2 mg/L NAA and MS +1.0 mg /L 6 -BA +0. 2 mg/L NAA, respectively.关键词
苹果/叶片培养/愈伤组织Key words
Apple/ Leaf culture/ Callus分类
农业科技引用本文复制引用
崔美,焦其庆,陈学森,陈晓流..苹果叶片愈伤组织的诱导培养[J].山东农业科学,2012,44(3):17-20,4.基金项目
山东省良种工程项目 （）
