山东医药2012，Vol.52Issue(9)：17-19,3.
细胞内酸化对自噬的影响
Influence of intracellular acidification on autophagy
摘要
Abstract
Objective To explore the influence of intracellular acidification on autophagy. Methods Using the pAd-miR-NHEl virus levitation liquid to infect SH-SY5Y cells, for the preparation of cell model of low NHE-1 gene, observing the formation of autophagy, changes of mitochondrial form and lysosome number of negative control group, the group after the succesful transfection 0.5, 2, 4, 8 h of adenovirus levitation liquid by transmission electron microscopy, testing the ex-pression of autophagy marker-LC3 by Western blotting. Results Through the transmission electron microscopy, the normal mitochondrial form and lysosome number were shown in negative control group. After the succesful transfection 0.5-8 h of adenovirus levitation liquid, swelling mitochondria, the lysosome populations, autophagy vesicles increased, the autophagy body formed. After 8 hours, apoptotic body appeared, mitochondria numbers decreased, no autophagy body, a bit lyso-somes. There was a low LC-3 expression in negative control group, after the succesful transfection 0.5 h, the expression of LC-3 rose (P <0.01) , then reached the peak in 4 h (P <0.01). In 8 h, the expression of LC-3 was lowest, it was lower than that in negative control group (P<0.01). Conclusions Cells acidification could activate the happening of autoph-agy.关键词
细胞内酸化/自噬/LC3Key words
cells acidification/ autophagy/ LC3分类
生物科学引用本文复制引用
肖楠,方伯言..细胞内酸化对自噬的影响[J].山东医药,2012,52(9):17-19,3.基金项目
国家自然科学基金资助项目(81100962) （81100962）
辽宁省博士科研启动、自然科学基金资助项目(20082179) （20082179）
辽宁省教育厅高等学校科研项目 (20060524) （20060524）
