山东医药2012，Vol.52Issue(10)：20-22,3.
大黄素对糖尿病KKAy小鼠PI3-K信号转导通路的影响
Effects of emodin on KKAy diabetic mouse in PI3-K signal transduction pathway
摘要
Abstract
Objective To observe the effects of emodin in type 2 diabetic KKAy mice blood glucose, insulin levels and phosphatidylinositol 3 - kinase ( PD-K ) signal transduction pathway. Methods 16 specific pathogen fiee(SPF) KKAy mice were divided randomly according to plasma glucose level into model group and treatment group, 8 normal C57BL/6J mice were selected as normal group. Mice of normal group and model group were fed with 20 ml/(d ? Kg) of sterile water, treatment group were given SO rag/kg emodin orally. 8 weeks later, all animals were tested fasting plasma glucose ( FPG) , fasting insulin level for caculating insulin sensitivity index (ISI), total triacylglycerol ( TG) , total cholesterol (TC). The expression of IRS-1 ,PB-K and Akt Ser473 in the musle tissue and adipose tissue were determined by Western blot. Results Compared with normal group, model group showed higher FBG, TC and TC, lower ISI( all P < 0.05 ) , and lower expression of IRS-1, PD-K and Akt Ser473 in the musle tissue and adipose tissue (all P < 0.05 ). Compared with model group, treatment group showed lower FBG, TG and TC higher ISI ( all P < 0.05). The expression of IRS-1, PI3-K and Akt Ser473 in the musle tissue and adipose tissue of treatment group is up-regulated as compared with model group (all P<0.05). Conclusion Emodin can increase the expression of IRS-1 ,PI3-K and Akt Ser473 in the musle tissue and adipose tissue, decrease FBG and improves insulin sensitivity of KKAy diabetic mice.关键词
大黄素/2型糖尿病/KKAy小鼠/胰岛素抵抗/磷脂酰肌醇3-激酶Key words
emodin/ type 2 diabetes mellitus/ KKAy diabetic mice/ insulin resistance/ phosphatidylinositol 3 kinase分类
医药卫生引用本文复制引用
宋冰,刘学政..大黄素对糖尿病KKAy小鼠PI3-K信号转导通路的影响[J].山东医药,2012,52(10):20-22,3.基金项目
辽宁省教育厅科学研究一般项目(L2011141). （L2011141）
