山东医药2012，Vol.52Issue(12)：70-72,3.
两种细胞增殖分析方法的比较性研究
Contrastive study of two cell proliferation assays
摘要
Abstract
Objective To compare the detection sensitivity between MTT and EdU incorporation for cell proliferation assay. Methods Prostate cancer cell line ( PC-3 ) was exposed to different concentrations of cisplatin for 24 hours. Cells viability and proliferation were respectively detected by MTT and EdU incorporation assay. Result PC-3 cell viability and proliferation were significantly decreased in 5 μg/mL condition by MTT detection (P <0. 05) , however, the proliferation positive cell ratio and the density of fluorescence were significantly decreased in 2 μg/mL condition by EdU incorporation assay ( P < 0.05). Conclusion Simple, fast and economic are the advantages of MTT method, but it only reflects the sta-tus of viable cells, independent with proliferation population; sensitivity, objectivity and specificity are the advantages of EdU incorporation assay, which is related with S phase cell cycle, directly reflects the cells proliferation.关键词
细胞增殖/MTT/EdUKey words
cell proliferation/ MTT/ EdU分类
医药卫生引用本文复制引用
韩建群,修瑞娟..两种细胞增殖分析方法的比较性研究[J].山东医药,2012,52(12):70-72,3.基金项目
北京协和医学院青年基金(imc-2010) （imc-2010）
公益性科研院所基本科研业务费专项基金(imc-2008). （imc-2008）
