山东医药2012，Vol.52Issue(14)：1-3,3.
结直肠癌组织中miR-338-3p、SMO的表达变化及意义
Expression and significance of miR-338-3p and SMO in colorectal carcinoma tissues
摘要
Abstract
Objective To investigate the expression and significance of microRNA(miRNA)-338-3p (miR-338-3p) and Smoothened (SMO) in colorectal carcinoma tissues. Methods The colorectal carcinoma and adjacent non-tumorous tissues were collected ( from 30 patients ) respectively and the total RNA and protein were extracted routinely. The miR-338-3p was detected by Real-time quantitative PCR. SMO mRNA and corresponding protein were detected by semi-quantitative RT-PCR and Western blot. Results In colorectal carcinoma tissues, the miR-338-3p, SMO mRNA and SMO protein was 0.153 7 ±0. 126 4, 0.371 ±0.116, 3.195 ±1.623, respectively, whereas in the adjacent non-tumorous tissues , the miR-338-3p, SMO mRNA and SMO protein was 0.901 5 ± 0.426 3, 0.366 ± 0. 117, 0. 733 ± 0. 305, respectively. The differeace between the expression of miR-338-3p and SMO protein in these two tissues was statistically significant ( all P <0.01). There was a remarkable inverse correlation between miR-338-3p and SMO protein levels was observed ( r = -0.877,P <0.01). Conclusion The expression of miR-338-3p was significantly down-regulated in colorectal carcinoma tissues, whereas the SMO protein was markedly increased. miR-338-3p may inhibit SMO expression by post-tran-scriptional gene silencing to restrain colorectal carcinoma occurrence and progress.关键词
结肠肿瘤/直肠肿瘤/微小RNA/Smoothened基因Key words
colonic neoplasms/ rectal neoplasms/ microRNA/ Smoothened gene分类
医药卫生引用本文复制引用
苏桂源,孙凯,李国新,余江..结直肠癌组织中miR-338-3p、SMO的表达变化及意义[J].山东医药,2012,52(14):1-3,3.基金项目
国家自然科学基金青年科学基金资助项目(81101896) （81101896）
广东省科技计划重点项目(2008A030201017) （2008A030201017）
广东省医学科研基金资助项目(B2010190). （B2010190）
