山东医药2012，Vol.52Issue(20)：7-9,3.
IDO基因转染宫颈癌细胞SiHa在体外抑制NK细胞杀伤作用的研究
IDO gene transfer in cervical cancer cell line SiHa inhibit the cytotoxic activity of natural killer cells in vitro
摘要
Abstract
Objective To investigate the effect of Indoleamine 2,3-dioxygenase (IDO J expression on the sensitivity of cervical cancer cells against natural killer ( NK) cells after IDO gene transfer. Methods SiHa cells were stably transfect-ed with IDO expression vector and Mock vector. Western blot analysis was used to detect IDO expression in SiHa, SiHa/ Mock and SiHa/IDO cells. Cell growth curves were performed using MTT kit. Sensitivity of SiHa, SiHa/Mock and SiHa/ IDO cells to NK cells were investigated by colorimetric assay using MTT. Results Only SiHa/IDO could express IDO. There was no intergroup difference in doubling time in vitro (P >0.05). The percent survival of SiHa/IDO cells was significantly higher than those of the control (SiHa and SiHa/Mock) cells (P<0.05). Conclusions IDO expression did not influence the cell growth of SiHa cells in vitro. IDO expression inhibited the cytotoxic activity of NK cells against cervical cancer cells. Therefore, IDO acts not only as a prognostic factor of cervical cancer but also as a potential therapeutic gene target against cervical cancer.关键词
IDO/子宫肿瘤/基因治疗/杀伤细胞,天然Key words
IDO/ uterine neoplasms/ gene therapy/ killer cells, natural分类
医药卫生引用本文复制引用
王冬冬,霍乃晨,王晓黎,张淑兰..IDO基因转染宫颈癌细胞SiHa在体外抑制NK细胞杀伤作用的研究[J].山东医药,2012,52(20):7-9,3.基金项目
辽宁省博士启动基金项目(20111108). （20111108）
