山东医药2012，Vol.52Issue(20)：10-12,15,4.
曲古抑菌素A对甲状腺鳞癌SW579细胞株p53和p21表达的影响
Effects of trichostostin A on the expression of p53 and p21 in thyroid squamous cell carcinoma cell line SW579
摘要
Abstract
Objective To observe the effects of triehostatin A (TSA) on the expression of p53 and p21 in thyroid squamous cell carcinoma cell line SW579 and lo explore its mechanism. Methods SW579 cells were cultured in vitro and divided into control, 50, 100, 200 and 400 nmol/L TSA groups randomly. The anti-proliferative effect of TSA against SW579 cells was detected by MTT. The p53 and p21 mRNA expression levels were determined by RT-PCR and p53 and p21 protein expression levels were detected by Western blot. Results The proliferation index decreased markedly with a dose-dependent manner. Compared with control groups, the expression levels of p21 mRNA and proteins of p21 and p53 were significantly elevated in various TSA groups, while p53 mRNA level was unchanged. Conclusion TSA can inhibit the proliferation of SW579 in a dose-dependent manner and increase p53 and p21 protein levels that may be the inhibit-pro-liferarion and induce-differentiation mechanism of TSA on thyroid squamous cell carcinoma cell line in vitro.关键词
曲古抑菌素A/甲状腺肿瘤/p53/P21/SW579Key words
trichostatin A/ thyroid neoplasms/ p53/ p21/ SW579分类
医药卫生引用本文复制引用
刘超,赵颂,张秀梅,王翠瑶,刘洋,肖建英..曲古抑菌素A对甲状腺鳞癌SW579细胞株p53和p21表达的影响[J].山东医药,2012,52(20):10-12,15,4.基金项目
辽宁省教育厅重点实验室项目(LS2010101). （LS2010101）
