上海农业学报2012，Vol.28Issue(2)：17-20,4.
鸭肝炎病毒Ⅰ型基因片段的原核表达及单克隆抗体的制备
Prokaryotic expression of gene segment of duck hepatitis virus Ⅰ and preparation of monoclonal antibody of DHV- Ⅰ
摘要
Abstract
A pair of primers were designed according to the Genebank-reported complete genome of duck hepatitis virus I (DHV-1) ,and a specific gene segment of DHV-1 was obtained by RT-PCR. The segment was cloned into the expression vector pET-28a,then the recombinant plasmids were transfected into Rosetta-gami(DE3) .induced by IPTG and purified by the Ni-NTA,and thus the recombinant fusion protein(20 kD or so)was obtained. The BALB/c mice were immunized with this recombinant protein,and a monoclonal antibody cell strain of DHV-1 was produced. The Western-blot analysis and ELISA showed that the recombinant protein of DHV-1 could react with DHV-1 positive serum and DHV-1 monoclonal antibody. Also the DHV- I monoclonal antibody could detect natural protein of DHV-1 through western-blot assay.关键词
鸭肝炎病毒Ⅰ型/原核表达/单克隆抗体Key words
Duck hepatitis virus Ⅰ/Prokaryotic expression/ Monoclonal antibody分类
农业科技引用本文复制引用
高骏,孙凤萍..鸭肝炎病毒Ⅰ型基因片段的原核表达及单克隆抗体的制备[J].上海农业学报,2012,28(2):17-20,4.基金项目
上海市科委科技创新行动计划(09140900300)资助 （09140900300）
