色谱2012，Vol.30Issue(3)：262-266,5.DOI:10.3724/SP.J.1123.2011.10035
固相萃取-气相色谱-串联质谱法测定水果中11种三唑类杀菌剂
Determination of 11 triazole fungicides in fruits using solid phase extraction and gas chromatographytandem mass spectrometry
摘要
Abstract
A method was developed and validated for the simultaneous determination of 11 tria-zole fungicides (tetraconazole, triflumizole, penconazole, hexaconazole, flutriafol, myclobu-tanil, etuconazole, propiconazole, tebuconazole, epoxiconazole and fluquinconazole) in fruits by gas chromatography-tandem mass spectrometry (GC-MS/MS). The triazole fungicides were extracted from the samples with acetonitrile, then enriched and cleaned-up with solid phase extraction (SPE) on a Carbon/NH2 cartridge (collecting 2-10 mL effluent). The detection was carried out by GC-MS/MS in the multiple reaction monitoring (MRM) mode, and the quantification analysis was performed by external standard method. The calibration curves showed good linearity in the range of 10 - 500 μg/L. The correlation coefficients were larger than 0. 994 0. The average recoveries of the 11 fungicides spiked in the fruits at the levels of 10, 50, 100 and 250 μg/kg were between 82.6% and 117. 1%, and the relative standard deviations (RSD) were less than 10%. The limits of quantification (S/N = 10) were between 0. 8 μg/kg and 3. 4 μg/kg. The method possesses low background, high sensitivity, and quantification limits lower than that of the national standard and the values reported in the relevant literature. It can be applied to the routine analysis of the 11 triazole fungicides in fruits.关键词
固相萃取/气相色谱-串联质谱/三唑类杀菌剂/水果Key words
solid phase extraction ( SPE)/ gas chromatography-tandem mass spectrometry (GC-MS/MS)/ triazole fungicide/ fruits分类
化学化工引用本文复制引用
李继革,王玉飞,施家威,蒋丽,姚浔平,方兰云..固相萃取-气相色谱-串联质谱法测定水果中11种三唑类杀菌剂[J].色谱,2012,30(3):262-266,5.基金项目
浙江省医药卫生科学研究基金项目(2011RCB032). （2011RCB032）
