色谱2012，Vol.30Issue(3)：298-303,6.DOI:10.3724/SP.J.1123.2011.11014
高效液相色谱-蒸发光散射检测法在烟用香精料液中水溶性糖及山梨醇分析中的应用
Application of high performance liquid chromatographyevaporative light scattering detection in determination of water-soluble sugars and sorbitol in tobacco flavourings and casings
摘要
Abstract
A simultaneous determination method of water-soluble sugars (rhamnose, xylose, fructose, glucose, sucrose, maltose) and sorbitol in tobacco flavourings and casings based on high performance liquid chromatography-evaporative light-scattering detection ( HPLC-ELSD) was established. The analytes were extracted with ultrasonic assisted extraction into water and cleaned-up by Isolute ENV + solid phase extraction (SPE) cartridge, and then separated on Prevail Carbohydrate ES column (250 mm×4. 6 mm, 5μm) with acetonitrile and water (75: 25, v/v) as mobile phase. The temperature of the drift tube in ELSD was 79 t and the flow rate of carrier gas of nitrogen was 2. 0 L/min. The calibration curves were plotted on the double logarithmic scales with the mass concentrations of analytes in the range of about 0. 06 - 1.2 g/L. The limits of detection (LODs) were in the range of 12 -26 mg/L. And the spiked recoveries in real samples were 88% - 109%. Based on the double logarithmic calibration of analytes, experiments were carried out to indicate that the physical and chemical characteristics of analytes , the retention behaviors on column and the elution ability of mobile phase can all influence the response of ELSD.关键词
高效液相色谱/蒸发光散射检测/双对数工作曲线/水溶性糖/山梨醇/烟用香精料液Key words
high performance liquid chromatography ( HPLC)/ evaporative light-scattering detection (ELSD)/ double logarithmic calibration curve/ water-soluble sugar/ sorbitol/ tobacco flavourings and casings分类
化学化工引用本文复制引用
胡斌,王昇,谢复炜,刘惠民..高效液相色谱-蒸发光散射检测法在烟用香精料液中水溶性糖及山梨醇分析中的应用[J].色谱,2012,30(3):298-303,6.基金项目
国家烟草专卖局科技攻关项目(No.110200601026). （No.110200601026）
