色谱2012，Vol.30Issue(3)：304-308,5.DOI:10.3724/SP.J.1123.2011.11015
毛细管电泳法分析藏红花植物细胞多糖中单糖组成
Analysis of monosaccharides in the saffron corm glycoconjugate by capillary electrophoresis
摘要
Abstract
The monosaccharides in the saffron corm glycoconjugate were separated by capillary electrophoresis ( CE) coupled with pre-column derivatization. 4-Methoxyaniline was used as derivatization reagent. The derivatization and CE separation conditions were investigated. The ultraviolet detection wavelength was 234 nm. The maximum yield of this derivatization reaction was obtained under the presence of 9.5% (v/v) acetic acid at 80 t for 2 h. An uncoated fused-silica capillary of 50 μm I. D. And 50/60 cm length (effective length/total length) was employed, and a pressure injection (3. 447 5 kPa, 5 s) was applied. The baseline separation of 11 monosaccharides and disaccharides (lyxose, xylose, ribose, glucose, mannose, galactose, rhamnose, cellobiose, maltose, lactose, fructose) was reached at 25 t, 20 kV of separation voltage and with 350 mmol/L boric acid (pH 10. 21) as running buffer. The developed method has been successfully applied to quantitatively determine the components of saffron corm glycoconjugate , and the results showed that the recovery of each monosaccharide was in the range of 94. 3% - 105. 4%, the relative standard deviation was 3. 3% -4. 6%.关键词
毛细管电泳/对甲氧基苯胺/柱前衍生/单糖/酮糖/多糖/藏红花Key words
capillary electrophoresis ( CE)/ 4-methoxyaniline/ pre-column derivatization/ monosaccharides/ ketoses/ glycoconjugate/ saffron分类
化学化工引用本文复制引用
马海宁,华玉娟,屠春燕,袁丽红,韦萍..毛细管电泳法分析藏红花植物细胞多糖中单糖组成[J].色谱,2012,30(3):304-308,5.基金项目
国家重点基础研究发展计划项目(2009CB724700). （2009CB724700）
