色谱2012，Vol.30Issue(3)：314-317,4.DOI:10.3724/SP.J.1123.2011.11018
高效液相色谱-串联质谱法测定蜂胶中的氯霉素
Determination of chloramphenicol in propolis by high performance liquid chromatographytandem mass spectrometry
摘要
Abstract
A method for the determination of chloramphenicol in propolis was developed by high performance liquid chromatography-tandem mass spectrometry. The flavones were removed with lead acetate solution after the extraction of the sample with water. The extract was cleaned up by liquid-liquid extraction. Internal standard method was used for quantitative analysis. The linear range was 0.05 - 2.0μg/L and the correlation coefficient ( r2 ) was 0. 999 6. The limit of detection (LOD, S/N = 3) and limit of quantitation (LOQ, S/N = 10) were 0. 1 μg/kg and 0. 3 μg/kg, respectively. The recoveries ranged from 70. 1% to 94.0% while the intra-day precision lower than 10% and inter-day precision lower than 15%. The method reduced the interference by removing most of the flavones and was suitable for the determination of chloramphenicol in propolis.关键词
高效液相色谱-串联质谱/氯霉素/蜂胶Key words
high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/ MS)/ chloramphenicol/ propolis分类
化学化工引用本文复制引用
张晓燕,陈惠兰,张睿,许蔚,黄娟,刘艳,吴斌,陈磊,丁涛,沈崇钰..高效液相色谱-串联质谱法测定蜂胶中的氯霉素[J].色谱,2012,30(3):314-317,4.基金项目
国家质量监督检验检疫总局科技项目(2012IK167). （2012IK167）
