胭脂鱼胰蛋白酶cDNA克隆以及不同蛋白含量日粮和饥饿对mRNA表达和酶活力的影响OA北大核心CSCDCSTPCD
MOLECULAR CLONING OF TRYPSIN AND THE EFFECT OF DIETARY PROTEIN LEVELS AND STARVATION ON TRYPSIN mRNA EXPRESSION AND ENZYME ACTIVITY IN JUVENILE CHINESE SUCKER (MYXOCYPRINUS ASIA TICUS BLEEKER)
为检测不同蛋白含量的日粮和饥饿对胭脂鱼(Myxocyprinus asiaticus)幼鱼胰蛋白酶活性和mRNA表达的影响,首先用RACE和PCR的方法从胭脂鱼幼鱼的肝胰脏组织中克隆得到胰蛋白酶cDNA全长,然后用半定量RT-PCR和酶活性检测方法分别检测了经不同蛋白含量日粮(酪蛋白含量分别为35％、45％和55％)投喂和饥饿处理后的胭脂鱼幼鱼的胰蛋白酶mRNA表达水平和胰蛋白酶活力的变化.结果显示,胭脂鱼胰蛋白酶cDNA全长为912 bp.投喂…查看全部>>
The effect of dietary protein and starvation on the expression of trypsin was evaluated in the Chinese sucker (Myxocyprinus asiaticus Bleeker).The complete trypsin cDNA was cloned from juvenile Chinese sucker pancreatic tissue by using RACE and PCR methods.We used semi-quantitative RT-PCR and enzymatic activity measurements to quantify mRNA expression and trypsin activity in fish that were either starved or fed differing levels of dietary casein (35％,45％ and…查看全部>>
郑凯迪;冯波;李云;李英文
西南大学动物科技学院,淡水鱼类资源与生殖发育教育部重点实验室,重庆400716温州医学院生物化学教研室,温州325035温州医学院健康与环境生态研究所,温州325035西南大学动物科技学院,淡水鱼类资源与生殖发育教育部重点实验室,重庆400716
生物科学
胭脂鱼蛋白水平胰蛋白酶活性饥饿胰蛋白酶mRNA
Chinese sucker (Myxocyprinus asiaticus Bleeker)Dietary proteinEnzymatic activityStarvationTrypsin mRNA
《水生生物学报》 2012 (1)
gp150蛋白调控盘基网柄菌发育相关基因功能的研究
9-17,9
the National Key Scientific Program of China (2009CB941200) the National Natural Science Foundation of China (30670226) the Scientific Research Foundation for the Returned Overseas Chinese Scholars Education Ministry of China (2006-331) Dgctoral and Post-doctoral Research Foundation of Southwest University (2005-05) China Three Gorges Corporation Research Project to Y.Li
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