水生生物学报2012，Vol.36Issue(2)：329-338,10.DOI:10.3724/SP.J.1035.2012.00329
集球藻对盐胁迫的生理适应及细胞结构变化
CHANGES ON CELLULAR STRUCTURES AND PHYSIOLOGICAL ADAPTATION OF PALMELLOCOCCUS SP.SUBJECTED TO SALT STRESS
摘要
Abstract
Salt stress is one of important ecological factors influencing survival of desert soil algae. Palmellococcus Chod. Is a kind of green algae widely distribute in biological soil crusts and is able to accumulate red pigment (astax-anthin) and oil drops, and the algae has displayed especial physiological nature and potential value of application. Currently, studies on physiology, cellular structures and pigment accumulations of Palmellococcus sp. Were relatively scarce. In this study, Palmellococcus sp., isolated from desert algal crusts, was selected for experiment material. Physiological effects of salt stress on the algal cells were investigated by measuring biomass (expressed as chlorophyll a), PS Ⅱ activity (FJFm), intracellular production of water-soluble proteins and saccharides, MDA contents as well as the activities of SOD and CAT. The results showed that salt stress resulted in an obvious decrease in biomass and PS Ⅱ activity of the treated cells compared to the control cells. It also indicated that intracellular production of water-soluble saccharide and water-soluble proteins were all comparatively greater in the treatment cells than the control cells. Moreover, salt stress had a significantly deleterious effect on membrane lipid superoxide as assessed by measurement of MDA content, and SOD and CAT activities also presented obvious increases as compared to the control. At the same time, it showed from this study that cellular structures and morphological characteristics of Palmellococcus sp. Were found to keep intact and clear in the control condition, and there was plentiful lipid body accumulation at growth ana-phase of the control culture. However, gradual destruction property in cellular structures were observed from Palmellococcus sp. Subjected to salt stress, and synchronously starch and lipid body accumulation were also detected in the treated cells. Furthermore, unintelligible cellular structures and disappearance of cellular organs were observed from Palmellococcus sp. Under salt treatment, and plasmolysis and vacuolization were appeared in the treated algal cells. The research results maybe provided us some significative findings for better understanding physiological adaptation, mor phological changes and pigment accumulation of Palmellococcus sp. When subjected to salt stress environment, and simultaneously provided us many experimental data for further researches and applications about the green algae.关键词
荒漠藻类/集球藻/盐胁迫/生理响应/细胞结构/适应机制Key words
Desert algae/Palmellococcus sp./Salt stress/Physiological responses/Cellular structures/Adaptation metabolism分类
农业科技引用本文复制引用
饶本强,张列宇,吴沛沛,李敦海,刘永定..集球藻对盐胁迫的生理适应及细胞结构变化[J].水生生物学报,2012,36(2):329-338,10.基金项目
中国博士后科学基金资助项目(20100480920) （20100480920）
淡水生态与生物技术国家重点实验室资助项目(2008FBZ21) （2008FBZ21）
武汉市科技局与内蒙古自治区发改委重大科技产业化专项(200720112031) （200720112031）
河南省高等学校青年骨干教师资助计划项目(2010GGJS-106) （2010GGJS-106）
信阳师范学院青年骨干教师资助计划 （）
信阳师范学院高层次人才科研启动基金项目资助 （）
