中国生态农业学报2012，Vol.20Issue(6)：746-751,6.DOI:10.3724/SP.J.1011.2012.00746
生物复混肥对土壤微生物群落功能多样性和微生物量的影响
Effects of bio-compound fertilizer on soil microbial community functional diversity and microbial biomass
摘要
Abstract
The characteristics of soil microbes are sensitive indicators for soil fertility. Fertilization could influence soil physio-chemical and biological properties. This study illustrated the functions of bio-compound fertilizers in maintaining good soil micro-ecological environment using the Biolog ECO micro-plate technique and CHCl3 fumigation-extraction method. The study investigated the effects of bio-compound fertilizer, organic-inorganic compound fertilizer and inorganic fertilizer at equal N, P and K amounts on functional diversities of soil microbial communities and microbial biomass under short-term potted corn experiment in greenhouse conditions. The six types of substances that composed Biolog ECO plate substrates included carbohydrates, carboxylic acids, amino acids, polymers, amines and aromatic chemicals. Principal Component Analysis (PCA) depicted different utilization forms of resource carbon by soil microbes. Average well-color development (AWCD), Shannon index (H) and richness index (S) of soil microbe communities were the highest in bio-compound fertilizer treatment. Bio-compound fertilizers obviously increased microbial source carbon utility, especially those of aromatic chemicals and carbohydrates. A significant difference existed in metabolic ability of soil microorganisms under different fertilizer treatments. Bio-compound fertilizer score was positive for the first principal component, while other treatments scores were negative. The determinant carbon sources for the differences were carbohydrates and carboxylic acids. During corn growth period, soil microbial biomass increased gradually and then stable with time. The amounts of soil microbial biomass C (333.21 mg·kg-1), N (53.02 mg·kg-1) and P (22.20 mg·kg-1) were highest under bio-compound fertilizer treatment. At the same nutrient rates, bio-compound fertilization significantly improved utilization rates of carbon source, richness indexes and functional diversities of soil microbial community. This also increased the amounts of soil microbial biomass C, N and P.关键词
生物复混肥/土壤碳源利用率/土壤微生物量/土壤微生物群落/功能多样性/BiologKey words
Bio-compound fertilizer, Utilization of soil carbon source, Soil microbial biomass, Soil microbial community, Functional diversity, Biolog分类
农业科技引用本文复制引用
邵丽,谷洁,张社奇,高华,秦清军..生物复混肥对土壤微生物群落功能多样性和微生物量的影响[J].中国生态农业学报,2012,20(6):746-751,6.基金项目
国家自然科学基金项目(40871119,41171203)和农业部"948"项目(2010-Z20)资助 （40871119,41171203）
