生态学杂志2012，Vol.31Issue(3)：670-675,6.
长江白甲鱼ITS2序列结构和群体遗传多样性
ITS2 structure and population genetic diversity of Onychostoma sima (Sauvage) in Yangtzi River
摘要
Abstract
Through the PCR amplification of the DNA extracted from the Onychostoma sima sam-ples collected from Yangtze River, the partial sequence of 5. 8S and 28S nuclear ribosomal RNA gene and the complete sequence of nuclear ribosomal internal transcribed spacer 2 (ITS2 ) were obtained. By using the software for DNA analysis, the population genetic diversity of 0. Sima in the Changshou Lake Rare Fish Breeding Center of Chongqing Fishery Sciences Research Institute and in the Fuling Fish Breeding Center was analyzed. The ITS2 contained 876 bp nucleotides, and the T, C, A, and G were averagely accounted for 22% , 32.5% , 29.6% , and 15. 8% , re-spectively. A total of 50 variable sites were found, including 40 transversions (Tv) and 10 tran-sitions (Ts). The Ts/Tv ratio (R) was 0.25. All the 40 0. Sima individuals collected were of 40 haplotypes, the haplotypic diversity (H) was 1.000, the average number of nucleotide differ-ences (K) was 5.978, and the nucleotide diversity (Pi) was 0.0682. Neutrality test and cluster analysis showed the two 0. Sima populations were not differentiated into a single population. The genetic divergence of the two populations was higher, and their germplasm resources had much higher quality.关键词
珍稀鱼类/白甲鱼/转录间隔子2/遗传多样性/资源保护Key words
rare fish/ Onychostoma sima/ internal transcribed spacer 2 (ITS2) / genetic diversi-ty/ resource conservation分类
生物科学引用本文复制引用
马跃岗,朱杰,袁万安..长江白甲鱼ITS2序列结构和群体遗传多样性[J].生态学杂志,2012,31(3):670-675,6.基金项目
2010重庆市科委基本科研业务费项目(CH001)、重庆市水产研究所项目(20090901)、教育部博士后基金项目(20080430853)资助. （CH001）
