生态学杂志2012，Vol.31Issue(6)：1473-1479,7.
壬基酚对波纹巴非蛤(Paphia undulata)内脏团毒性效应
Toxic effect of nonylphenol on Paphia undulata visceral mass
摘要
Abstract
A semi-static toxicity test was conducted to determine the 96-h median lethal concentration (96-h LC50 value) of nonylphenol (NP) to Paphia undulata. In the meantime, the variations of superoxide dismutase (SOD), glutathione (GSH), and peroxidase (POD) activities and malondialdehyde (MDA) content in the visceral mass of P. undulata were studied when exposed to different concentrations NP (low: 1 μg· L-1 ; medium: 10 μg · L-1 ; and high; 25 μg·L-1 ) and during the recovery stage in clear sea water. The 96-h LC50 value was 260 μg· L-1 . At the early stage of stress, the SOD activity in the visceral mass of P. undulata exposed to low and medium NP concentration was inhibited (P<0.01) while the POD activity was induced (P<0.01), showing the typical "excitotoxicity effect". During the stress period, the SOD activity and GSH content in the visceral mass at low and medium NP concentrations increased after an initial decrease , while the POD activity and MDA content were in adverse. When exposed to high NP concentration, the SOD activity was inhibited first and induced then, the POD activity and MDA content increased after an initial decrease, while the GSH content was significantly higher than that of the control level in whole exposure time. The GSH and MDA contents in whole exposure time appeared sharp fluctuation, and the variation increased with increasing NP concentration. During recovery period, all the test indices in the visceral mass of P. undulata exposed to low and medium NP concentration recovered gradually to the normal level, but, for the P. undulata exposed to high NP concentration, these indices remained significant difference, as compared to the control group. The above results indicated that NP had obvious impact on the antioxidant system in the visceral mass of P. undulata, while P. undulata had self-repairing ability for the oxi-dative damage caused by certain degree of NP stress, but, at high NP concentration, the lipid peroxidation damage could not recover to the level of the control group in a short time.关键词
壬基酚/波纹巴非蛤/内脏团/96 h半致死浓度/抗氧化防御系统Key words
nonylphenol/ Paphia undulata/ visceral mass/ 96-h LC50/ antioxidant defense system.分类
生物科学引用本文复制引用
巩秀玉,陈海刚,张喆,马胜伟,黄志斐,王贺威,王涛,蔡文贵..壬基酚对波纹巴非蛤(Paphia undulata)内脏团毒性效应[J].生态学杂志,2012,31(6):1473-1479,7.基金项目
广东省科技计划项目(2009B030600001,2011A06092006)、中央级公益性科研院所基本科研业务费专项资金项目(2009YD01,2010YD04,2010TS12)、广东省自然科学基金博士启动项目(S2011040005251)和中国水产科学研究院基本科研业务费专项课题(2012A0203)资助. （2009B030600001,2011A06092006）
