生物加工过程2012，Vol.10Issue(2)：68-72,5.DOI:10.3969/j.issn.1672-3678.2012.02.014
多孔硅Bragg反射镜适配子生物传感器检测心肌肌钙蛋白Ⅰ
Detection of cardiac troponin Ⅰ based on aptamer biosensor on nano-porous silicon Bragg reflector
摘要
Abstract
Nano-porous silicon Bragg reflectors were fabricated using an electrochemical etching process. Then, the cTnI aptamers by binded with cTnI were immobilized to the porous silicon Bragg reflector surface using a crosslink method. After the binding between aptamers and the cTnI of different concentrations, the reflection spectrum red shifts of the porous silicon Bragg reflector were analyzed quantitatively. The result showed that the porous silicon Bragg reflector aptamer biosensor had high specificity. Furthermore,the procedures were label-free and detection time was reduced. The linear detection range of the sensor was 0. 05 -4 nmol/L, and the lowest detected concentration was 0. 05 nmol/L.关键词
适配子/生物传感器/多孔硅/Bragg反射镜/心肌肌钙蛋白ⅠKey words
aptamer/ biosensor/ porous silicon/ Bragg reflector/ cardiac troponin I分类
医药卫生引用本文复制引用
曾志鹏,张娟琨,刘珺,王明伟,林青,徐慧静..多孔硅Bragg反射镜适配子生物传感器检测心肌肌钙蛋白Ⅰ[J].生物加工过程,2012,10(2):68-72,5.基金项目
科技部资助项目(2009GJA10047) （2009GJA10047）
天津市科委资助项目(07ZHZHNC04500,09ZCZDSF04200) （07ZHZHNC04500,09ZCZDSF04200）
