生物信息学2012，Vol.10Issue(1)：8-11,4.DOI:10.3969/j.issn.1672-5565.2012.01.03
酿酒酵母SNF4基因的克隆及生物信息学分析
Cloning and bioinformatics analysis of SNF4 gene in saccharomycescerevisiae
摘要
Abstract
Snf4p is encoded by SNF4 gene which the function is regulating the protein kinase activity of Snfl complex. Clone SNF4 gene from S. Cerevisiae YS2 with primer which is designed by the known SNF4 sequence. Sequence analysis showed that SNF4 gene contains 969bp nucleotide acids, encoding 322 amino acids. Predict the physico - chemical property, hydrophobicity, signal peptide, subcellular localization, active site and spatial structure of SNF4 gene. The results show that Snf4p is the intracellular stability of acidic protein but does not exist transmembrane. The functional domain of Snf4p is CBS pair superfamily and the main secondary structure isa - helix. The spatial structure of Snf4p is dimer which is made by four CBS structural domains. Thephairpin structure reaction region between Snflp and Sip2p isplamellar structure which is in the first CBS pair of Snf4p.关键词
SNF4/酿酒酵母/ADH2/生物信息学Key words
SNF4/ Saccharomyces cerevisia / ADH2/ Bioinformatics分类
化学化工引用本文复制引用
吴毕莎,柯崇榕,林晓华,林清强,陈由强,黄建忠..酿酒酵母SNF4基因的克隆及生物信息学分析[J].生物信息学,2012,10(1):8-11,4.基金项目
现代农业产业技术体系建设专项资金资助(No.nycytx-024-01-20),公益性行业(农业)科研专项项目(No.nyhyzx07-019),农业部948项目(No.2006-G37),福建省教育厅项目(No.JB10015). （No.nycytx-024-01-20）
