生物信息学2012，Vol.10Issue(1)：65-70,6.DOI:10.3969/j.issn.1672-5565.2012.01.16
甘蔗过氧化氢酶基因的电子克隆及生物信息学分析
Electronic cloning and characterization of CAT gene from Saccharum officinarum using bioinformatics tools
摘要
Abstract
A catalase gene from Saccharum officinarum, termed as S - CAT, was cloned in silico based on the corresponding EST sequences from sugarcane EST database in NCBI. Several characters of the amino acid encoded by S - CAT gene were analyzed and predicted by bioinformatics tools, including the composition of amino acid sequence, hydrophobicity and hydrophilicity, secondary structure of protein and so on. Bioinformatics analysis also revealed that the full - length of S - CAT gene from S. Officinarum was 2 160 bp, containing a complete ORF encoding 492.amino acids. Using PSORT, it was predicted that the S - CAT protein was located in peroxisome and belonged to the CAT - POD type of catalases. Homology comparison and phylogenetic analysis showed that the amino acid encoded by S - CAT gene in sugarcane was highly homologous with those encoded by CAT gene in rice, maize, sorghum, Korean alkaligrass and grape, with homologies of 97 % , 97 % 95 % , 91 % and 92 % , respectively. The above results will provide tHe basis for molecular cloning of CAT genes in;sugarcane.关键词
甘蔗/CAT基因/电子克隆/生物信息学Key words
Saccharum officinarum/ Catalase gene/ In silico cloning/ Bioinformatics分类
生物科学引用本文复制引用
陈珊珊,郭晋隆,李国印,阙友雄,许莉萍..甘蔗过氧化氢酶基因的电子克隆及生物信息学分析[J].生物信息学,2012,10(1):65-70,6.基金项目
现代农业产业技术体系专项资金(CARS-20)、国家863计划课题(F2007AA100701)、国家自然科学基金(31101196). （CARS-20）
