山西大学学报（自然科学版）2012，Vol.35Issue(1)：85-88,4.
3,5-二硝基水杨酸与牛血清白蛋白相互作用的荧光光谱研究
Spectra Study on the Interaction of 3,5-Dinitrosalicylic Acid with Bovine Albumin
摘要
Abstract
The interaction between 3?5-dinitrosalicylic acid and bovine serum albumin were studied by fluorescence spectroscopy. The results shown that the quenching mechanism of the combination of bovine serum with 3,5-Dinitrosalicylic acid was a static quenching procedure. The number of binding sites was 1. The synchronous fluorescence indicated that the binding site of DNS with BSA was near by tryptophan subunit. The binding distance between 3,5-dinitrosalicylic acid with bovine serum albumin and the energy transfer efficiency were obtained based on the theory of Forster spectroscopy energy transfer.关键词
3,5-二硝基水杨酸/牛血清白蛋白/荧光光谱/同步荧光Key words
3,5-Dinitrosalicylic acid/ bovine serum/ fluorescence spectroscopy/ synchronous fluorescence分类
化学化工引用本文复制引用
王志军,谢晶,雷海英,梁瑞瑞..3,5-二硝基水杨酸与牛血清白蛋白相互作用的荧光光谱研究[J].山西大学学报（自然科学版）,2012,35(1):85-88,4.基金项目
山西省高校科技开发项目(20081042) （20081042）
山西省高等学校大学生创新创业训练项目(2011362) （2011362）
长治学院校级资助项目(2011116) （2011116）
