山西大学学报（自然科学版）2012，Vol.35Issue(1)：118-121,4.
Periaxin蛋白中PDZ结构域的重组表达与纯化
Recombinant Expression and Purification of Periaxin-PDZ Domain
摘要
Abstract
Periaxin is a protein of noncompact myelin in the peripheral nervous system. The loss-of-f unction mutation of the gene encoding periaxin can cause demyelinating Charcot-Marie-Tooth 4F disease. The gene of periaxin-PDZ was amplified by PCR with pBluescrip+SK( Ⅱ )-periaxin as the template and inserted into the vector pETM-3C to form a recombinant plasmid pETM-3C-pdz. The recombinant protein was overex-pressed in Escherichia coli BL2KDE3) by IPTG induction. A purification procedure for periaxin-PDZ was performed by Ni2+-NTA column and Sephacryl S-200 column. The lipid membrane binging capacity of the Periaxin PDZ domain was evaluated with fluorescence spectroscopy. The results show that dissociation constants of lipid membranes with Periaxin-PDZ (18-102aa) or Periaxin-PDZ( 18-129aa) is 4. 415 μg/mL or 7. 265 μg/mL respectively. The lipid membrane binging capacity of Periaxin-PDZ(18-102aa)is slightly higher than the extended Periaxin-PDZ(18-129aa).关键词
腓骨肌萎缩症/Periaxin蛋白/PDZ结构城/脂质体/荧光光谱法Key words
Charcot-Marie-Toothdisease/ Periaxin/ PDZdomain/ liposome/ fluorescence spectroscopy分类
生物科学引用本文复制引用
石亚伟,任燕,刘俊林,杨廷..Periaxin蛋白中PDZ结构域的重组表达与纯化[J].山西大学学报（自然科学版）,2012,35(1):118-121,4.基金项目
国家自然科学基金(31170748) （31170748）
生物大分子国家重点实验室2010年开放课题 （）
