山西医科大学学报2012，Vol.43Issue(5)：321-324,397,5.DOI:10.3969/J.ISSN.1007-6611.2012.05.001
血清学指标在兔肝纤维化模型中的实验研究
Experimental study on serum fibrosis biomarkers in hepatic fibrosis rabbit model
摘要
Abstract
Objective To explore the relationship between serum fibrosis biomarkers and stages of hepatic fibrosis. Methods Forty-five rabbits were randomly divided into experiment group( n = 36 ) and control group( n = 9 ). Hepatic fibrosis models were established by intraperitoneal injection of carbon tetrachloride( CCl4 ) in experiment rabbits. Serum fibrosis biomarkers( hyaluronic acid, type Ⅲ procollagen,laminin,type Ⅳ collagen ) and pathology were observed respectively at the 6th,9th and 12th week after the first injection. According to pathological grade and stage, the rabbits were grouped. Results Thirty rabbits completed the detection, including 9 cases of normal liver,6 cases of mild liver fibrosis ,7 cases of moderate liver fibrosis and 8 cases of severe liver fibrosis. With the aggravation of hepatic fibrosis,hyaluronic acid,type Ⅲ procollagen,laminin,type Ⅳ collagen were increased gradually. All those four biomarkers were positively correlated with histopathological stagings P < 0. 05 ), especially type Ⅳ collagen ( r=0.718,P<0.05 ). Conclusion Serum fibrosis biomarkers are effective indices to evaluate the severity of hepatic fibrosis. Ⅳ-C is the best for the diagnosis of hepatic fibrosis in the four biomarkers.关键词
肝纤维化/兔/动物模型/血清标志物Key words
hepatic fibrosis/rabbits/animal model/serum biomarkers分类
医药卫生引用本文复制引用
彭婕,冯菲,潘桂霞,彭烨,李晓栋,张桉瑜,董爱生,左长京..血清学指标在兔肝纤维化模型中的实验研究[J].山西医科大学学报,2012,43(5):321-324,397,5.基金项目
国家自然科学基金青年科学基金资助项目(81000601) （81000601）
