山西医科大学学报2012，Vol.43Issue(5)：337-340,398-399,6.DOI:10.3969/J.ISSN.1007-6611.2012.05.005
P2X7受体拮抗剂A438079对肠易激综合征结肠扩张刺激大鼠DCN核团中P2X7、OX42、IL-1β、P38及骶髓后角中CGRP表达的影响
Effect of P2X7 receptor antagonist A438079 on expression of P2X7,OX42,IL-1β,P38 in dorsal commissural nucleus and CGRP expression in dorsal horn of sacral segment of spinal cord in rats with irritable bowel syndrome
摘要
Abstract
Objective To explore the effect of A438079, a P2X7 receptor antagonist, on expression of P2X7 , 0x42, IL-1 β, P38 in dorsal commissural nucleus and calcitonin gene related peptide( CGRP ) in dorsal horn of sacral segment of spinal cord in rats with irritable bowel syndrome ( IBS ) induced by the stimulation of colorectal distention, and to provide a theoretical evidence in the prevention and treatment of IBS. Methods Fifteen rats were gavaged with the Trichinella spiralis to establish the IBS model, and then were divided into three groups: IBS + colon distension group ( B ), IBS + colon distension + intrathecal injection of saline ( C ), and IBS + colon distension + intrathecal injection of A438079( D ). Five normal rats with colon distension were chosen as control group. Immunofluorescent staining method was used to observe the expression of P2X7,OX42,I L-1β,P38 in neurons of DCN and CGRP expression in dorsal horn of the sacral segment of spinal cord in rats. Results The expression of P2X7, 0X42, IL-1 β, P38 and CGRP in IBS + colon distension + A438079 group were all significantly lower than those in IBS + colon distension group ( P <0. 01 ). Conclusion P2X7 receptor may be involved in the development of IBS-induced visceral hyperalgesia.关键词
肠易激综合征/内脏敏化/小胶质细胞/A438079/P2X7受体/IL-1βKey words
irritable bowel syndrome/visceral hyperalgesia/microglia cells/A438079/P2X7 receptor/IL-1β分类
医药卫生引用本文复制引用
朱琳,章鹏宇,黄裕新,王景杰..P2X7受体拮抗剂A438079对肠易激综合征结肠扩张刺激大鼠DCN核团中P2X7、OX42、IL-1β、P38及骶髓后角中CGRP表达的影响[J].山西医科大学学报,2012,43(5):337-340,398-399,6.基金项目
国家自然科学基金资助项目(30871142) （30871142）
