山西医科大学学报2012，Vol.43Issue(6)：424-428,5.DOI:10.3969/J.ISSN.1007-6611.2012.06.006
神经外科病房金黄色葡萄球菌的耐药性及MRSA基因多态性研究
Drug resistence and genetic polymorphism of methicillin-resistant Staphylococcus aureus in department of neurosurgery
雷金娥 1韩蕾 2陈艳炯 2刘军辉 1沈燕芳 2张凯歌 1曾晓艳1
作者信息
- 1. 西安交通大学医学院第一附属医院检验科,西安,710061
- 2. 西安交通大学医学院微生物学与免疫学系
- 折叠
摘要
Abstract
Objective To investigate the drug resistance of Staphylococcus aureus and genetic polymorphism of methicillin resistant Staphylococcus aureus ( MRSA) in a department of neurosurgery of a hospital. Methods Antibiotic resistance of 254 strains isolated from 2008 —2011 in the department of neurosurgery was determined by K- B disk diffusion MRSA was identified by cefoxitin of disk diffusion and double-PCR of mecA and femA gene. Randomly amplified polymorphic DNA ( RAPD) was applied to analyze the homology of MRSA strains. Results The prevalence of MRSA was 92. 9%. All isolated strains were susceptible to vancomycin , teicoplanin and linezolid. The susceptibility to trimethoprim/sulfamethoxazole and chloramphenicol was 82. 3% and 74.4% ,respectively. The resistant rates against levofloxacin ,erythromycin,ciprofloxacin and penicillin were higher than 95% ,respectively. Four different RAPD patterns were found among 32 strains in the department of neurosurgery ,and most of MRSA was type I (62.5% ). Conclusion Type I MRSA outbreak exists in the neurosurgery ward of the hospital.关键词
耐甲氧西林金黄色葡萄球菌/随机引物多态性DNA/耐药性Key words
meticillin -resistant Staphylococcs aureus / randomly amplified polymorphic DNA / drug resistance分类
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雷金娥,韩蕾,陈艳炯,刘军辉,沈燕芳,张凯歌,曾晓艳..神经外科病房金黄色葡萄球菌的耐药性及MRSA基因多态性研究[J].山西医科大学学报,2012,43(6):424-428,5.
