现代检验医学杂志2012，Vol.27Issue(1)：24-27,4.DOI:10.3969/j.issn.1671-7414.2012.01.008
两种肠系膜淋巴液处理方法的确立及探讨
Establishment and Discussion the Two Methods of Processing Mesenteric Lymph
摘要
Abstract
Objective To research the role of the mesenteric lymph fluid in "gut-lymph pathway" during rat intestinal ische-mia-reperfusion (I/R) injury,establish and discuss the methods of processing mesenteric lymph fluid. Methods 20 healthy male rats of SPF grade randomly was divided into 2 groups: intestinal ischemia-reperfusion+ drainage group (I/R+D) and normal drainage (N+D) group. The rats in I/R+D group were subjected to 60 min ischemia by clapping the superior mesenteric artery,followed by 120 min reperfusion. At the same time, the rats in I/R + D group were treated with intestinal lymph drainage for 180 min. Rats in N + D group were only treated with intestinal lymph drainage for 180 min. Collected the mesenteric lymph fluid of the two groups during the 180 min. The processing lymph fluid of two groups as followed :①Pro-teinase K degrades the protein of two groups,before and after that,detected the protein concentration. ②Endotoxin removing ' columns bind and remove pyrogens from the lymph,detecting the endotoxin before and after the processing. Results Found that the levels of protein concentration and endotoxin of lymph fluid were significant higher than those of control group after intestinal (I/R) injury (t value were -8. 32 and -9. 91 separately, P<0. 05 was considered statistically significant). Pro-teinase K could degrade the protein of two groups:N+D (24. 35 + 1. 37)g/L vs (2. 94 ± 0. 33)g/L,I/R + D(34. 89 ± 1. 68) g/I. Vs (3. 72 + 0. 51) g/L,efficiency up to 87% ; Endotoxin removing columns could bind and removed pyrogens from the lymph: N + D(0.009 5±0. 005 3) EU/ml vs (0.002 1 + 0.000 5) EU/ml,I/R+D(0. 032 l±0. 013 1) EU/ml vs (0.003 0 + 0. 000 3) EU/ml,efficiency ups to 90% in I/R+D group. Conclusion The levels of protein concentration and endotoxin of mesenteric lymph fluid were much higher than the normal control after intestinal (I/R) injury. Proteinase K and endotoxin removing columns could remove the most of the protein and endotoxin from the lymph. That could be a new method for further research the role of the mesenteric lymph fluid during the intestinal (I/R) injury.关键词
肠道缺血再灌注/肠系膜淋巴液/肠淋巴液处理/淋巴液蛋白浓度/淋巴液内毒素Key words
intestinal ischemia reperfusion/mesenteric lymph fluid/mesenteric lymph processing/lymph concentration of protein/ lymphconcentration of endotoxin分类
医药卫生引用本文复制引用
张睿,何桂珍,王玉康..两种肠系膜淋巴液处理方法的确立及探讨[J].现代检验医学杂志,2012,27(1):24-27,4.基金项目
北京自然科学基金资助项目(7102127). （7102127）
