现代检验医学杂志2012，Vol.27Issue(1)：49-51,3.DOI:10.3969/j.issn.1671-7414.2012.01.016
EHD2下调促进乳腺上皮细胞转化的研究
Down-regulation of EHD2 Enhanced Transformed Growth of Breast Epithelial Cell
摘要
Abstract
Objective To study the effects of EHD2 down-regulation on breast epithelial cell transformation. Methods Western Blot was used to analyse the EHD2 expression in breast invasive ductal carcinoma. Used soft agar colony formation assay to test the effect of EHD2 down-regulation on cell transformation. Results The EHD2 protein expression level was significantly lower in cancer tissue(F=5. 389, P<0. 05) ,Down-regulation of EHD2 was more clearly by siRNA(t=101. 458, P< 0. 005). The potency of transformed cell growth was significantly increased (t= 88. 754, P<0. 000). Conclusion EHD2 would be a novel breast tumor suppressor, and its impaired expression could cause breast epithelial cell transformation.关键词
EHD2/乳腺癌/转化Key words
EHD2/breast cancer/ transformation分类
医药卫生引用本文复制引用
田刚,王学涵,李盼,王洪玉,郭立莎,肖春花,应国光..EHD2下调促进乳腺上皮细胞转化的研究[J].现代检验医学杂志,2012,27(1):49-51,3.基金项目
本研究项目受国家自然科学基金(编号:30772528,81072158)和天津市滨海新区卫生局科研基金(编号:2100BHKL004,2100BHKY023)资助. （编号:30772528,81072158）
