现代检验医学杂志2012，Vol.27Issue(1)：99-100,103,3.DOI:10.3969/j.issn.1671-7414.2012.01.031
流式细胞术在检测细胞内活性氧中的应用
Application of Flow Cytometry in Detection of Cellular Reactive Oxygen Species
摘要
Abstract
Objective To detect the levels of reactive oxygen species (ROS) of hippocampad neurons cells by flow cytometry. Methods Mouses were randomly divided into 3 groups(15 cases in experiment group,5 cases in positive control group and 5 cases in negative control group). Cells were treated with blueberry anthocyanins and H2O2 before incubating with (DCF-DA). The fluorescent intensity of cellular DCF was detected by flow cytometry. Negative controls and positive controls were also set up. Results Anthocyanins could reduce intracellular ROS generation induced by H2 O2. Conclusion The method by flow cytometry to measure the reactive oxygen species is simple, reliable and stable.关键词
流式细胞术/活性氧/二氯荧光素/双乙酸盐(DCF-DA)/蓝莓花色苷Key words
cytometry/reactive oxygen species/dichlorofluorescein diacetate(DCF-DA) / blueberry anthocyanins分类
医药卫生引用本文复制引用
赵凯,陈琦,王利,贾宇臣..流式细胞术在检测细胞内活性氧中的应用[J].现代检验医学杂志,2012,27(1):99-100,103,3.基金项目
内蒙古医学院科技百万基金项目(NY2011BW002). （NY2011BW002）
