实用妇产科杂志2012，Vol.28Issue(2)：108-111,4.
重组蛋白LHRH-PE40对卵巢癌细胞增殖与凋亡的影响
Effect of Recombinant Toxin LHRH-PE40 on Proliferation and Apoptosis of Ovarian Cancer Cell
韩欣悦 1向梅 1张茜 2朱平 3刘玉梅4
作者信息
- 1. 吉林大学第二医院,吉林长春130041
- 2. 山西省长治市第二医院,山西长治047100
- 3. 吉林省长春市基因工程药物研究所,吉林长春130062
- 4. 吉林省抚松县人民医院,吉林抚松134500
- 折叠
摘要
Abstract
Objective;To study the effects of recombinant protein LHRH-PE40 on ovarian cancer cell proliferation and apoptosis. Methods proliferation inhibition assay was taken in vitro cultured ovarian cancer SK cell. Different concentration of LHRH-PE40 was added into cell culture solution. The effects of LHRH-PE40 on ovarian cancer cell proliferation were analyzed by enzyme micro-plate reader 1-4 days after adding LHRH-PE40. The effects of LHRH-PE40 on proliferative factor Ki-67, anti-apoptosis gene Bcl-2 and cell cycle in ovarian cancer SK cells were detected by immunohistochemical staining and Cytometry. Results: LHRH-PE40 could inhibit the growth of in vitro cultured epithelial ovarian cancer SK cell, which presented time and concentration dependency. The best action time was 48-72 hours after administration of drugs. Under the concentration of medicine ipo/ml, the positive cell rate of Ki-67 (the rate before using medication was 73%, after was 25%) and Bcl-2 (the rate before using medication was 92 %, after was 43%) was decreased after medication, and the ratio of apoptosis was increased (from 0.8% to 3.0%). Under 0.5 ng/ml concentration, the cell ratio of Go/G1 was also increased, in which the cell ratio of S phase was increased and the ratio of G2/M decreased. Conclusions:LHRH-PE40 can kill ovarian cancer cells and promote apoptosis of ovarian cancer cell.关键词
LHRH-PE40/卵巢癌/抑制/Ki-67/Bcl-2Key words
LHRH-PE40/ Ovarian cancer/ Suppression/ Ki-67/ Bcl-2分类
医药卫生引用本文复制引用
韩欣悦,向梅,张茜,朱平,刘玉梅..重组蛋白LHRH-PE40对卵巢癌细胞增殖与凋亡的影响[J].实用妇产科杂志,2012,28(2):108-111,4.
