实用肝脏病杂志2012，Vol.15Issue(3)：203-205,3.DOI:10.3969/j.issn.1672-5069.2012.03.010
酒精培养C3A细胞NOTCH信号通路的表达
Notch signaling pathway expression in C3A cell line cultured with ethanol
摘要
Abstract
Objective To study the expression of Notch signaling pathway influenced by ethanol in C3A cells in vitro. Methods C3A cells were served as the control group,and the CYP2E1 cells were as experiment group. Equal concentration of ethanol and Notch signaling pathway inhibitor were added to the two groups of cells. RT-PCR and Western blotting were used to testify the expression of Notchl.NICDl and HES1 .respectively. MTT assay was used to evaluate the viability of cells. Results The cell viability in the two groups was decreased by ethanol,while the Notch signaling pathway inhibitor improved the viability of cells; The expression of NICD1 and HES1 was up-regulated in the experimental group,while when Notch signaling inhibitor was added into the medium, the expression of NICD1 and HES1 was significantly down-regulated; The Notch 1 mRNA level was also up -regulated in the experimental group. Conclusion Ethanol can activate the expression of Notch signaling pathway in C3A models.关键词
C3A细胞/酒精/Notch信号通路Key words
C3A cells/Ethanol/Notch signaling pathway引用本文复制引用
谢俏,李汇华,李鑫,徐有青..酒精培养C3A细胞NOTCH信号通路的表达[J].实用肝脏病杂志,2012,15(3):203-205,3.基金项目
北京市自然科学基金资助项目(编号:7112048) （编号:7112048）
