微波辐射非热效应诱导人肺癌A549细胞自噬的研究OACSTPCD
Effect of autophagy on lung cancer cell line A549 by microwave radiation non-thermal effect
目的 探讨微波辐射非热效应对人肺癌A549细胞自噬的影响.方法 用冰浴法制备微波辐射非热效应模型,剂量为100 W-12 mV/cm2、150 W-18 mV/cm2和200 W-21 mV/cm2,照射10分钟；24小时后,免疫印记法检测细胞自噬相关蛋白Beclin-1、LC3的变化,泛素化蛋白表达变化；间接免疫荧光法检测自噬标志蛋白LC3和p62的变化.结果 微波辐射非热效应作用A549细胞,导致自噬相关蛋白Beclin-1表达增加,LC3和…查看全部>>
Objective To investigate the effect of autophagy induced by microwave radiation non-thermal effect on human A549 cell line. Methods Ice bath was employed to establish microwave radiation non-thermal effect model, and dosages were 100 W-12 mV/cm2, 150 W-18 mV/cm2 and 200 W-21 mV/cm2 for 10 minutes. After 24 hours, Western blot was used to detect the expression of Beclin-1, LC3 and ubiquitinated proteins. Indirect immunofluorescence was used to dectect the exp…查看全部>>
曹慧玲;徐冶;刘晓冬;于洋;刘师兵;王晓军;孙路阳
吉林医药学院医学科研实验室,吉林吉林132013吉林医药学院医学科研实验室,吉林吉林132013吉林医药学院医学科研实验室,吉林吉林132013吉林医药学院医学科研实验室,吉林吉林132013吉林医药学院医学科研实验室,吉林吉林132013吉林医药学院医学科研实验室,吉林吉林132013吉林医药学院医学科研实验室,吉林吉林132013
医药卫生
肺肿瘤微波/辐射效应自噬Beclin-1蛋白免疫印迹法
lung neoplasms microwaves/radiation effects autophagy Beclin-1 protein immunoblotting
《实用肿瘤杂志》 2012 (3)
271-273,3
吉林省教育厅科学技术研究资助项目(吉教科合字[2010]第253号)
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