实用肿瘤杂志2012，Vol.27Issue(3)：271-273,3.
微波辐射非热效应诱导人肺癌A549细胞自噬的研究
Effect of autophagy on lung cancer cell line A549 by microwave radiation non-thermal effect
摘要
Abstract
Objective To investigate the effect of autophagy induced by microwave radiation non-thermal effect on human A549 cell line. Methods Ice bath was employed to establish microwave radiation non-thermal effect model, and dosages were 100 W-12 mV/cm2, 150 W-18 mV/cm2 and 200 W-21 mV/cm2 for 10 minutes. After 24 hours, Western blot was used to detect the expression of Beclin-1, LC3 and ubiquitinated proteins. Indirect immunofluorescence was used to dectect the expression and location of the LC3 and p62 proteins. Results The non-thermal effect of microwave radiation on human A549 cells could increase the expression of Beclin-1, and induced activation of LC3 and p62. Conclusion The non-thermal effect of microwave radiation on human A549 cells could induce autophagy.关键词
肺肿瘤/微波/辐射效应/自噬/Beclin-1蛋白/免疫印迹法Key words
lung neoplasms/ microwaves/radiation effects/ autophagy/ Beclin-1 protein/ immunoblotting分类
医药卫生引用本文复制引用
曹慧玲,徐冶,刘晓冬,于洋,刘师兵,王晓军,孙路阳..微波辐射非热效应诱导人肺癌A549细胞自噬的研究[J].实用肿瘤杂志,2012,27(3):271-273,3.基金项目
吉林省教育厅科学技术研究资助项目(吉教科合字[2010]第253号) （吉教科合字[2010]第253号）
