生殖医学杂志2012，Vol.21Issue(2)：158-161,4.DOI:10.3969/j.issn.1004-3845.2012.02.017
线粒体DNA拷贝数对卵母细胞受精结局的影响
Mitochondrial DNA copy number effects fertilization outcome of oocytes
摘要
Abstract
Objective:To determine the effect of mitochondrial DNA (mtDNA) copy number on fertilization outcome of oocytes in order to provide new idea for improving fertilizing rate.Methods:The mtDNA copy number in fertilized and unfertilized oocytes and embryos were analyzed by real-time polymerase chain reaction (PCR).Results:There were 52 unfertilized oocytes in group A, 46 fertilized oocytes with pronucleus blocking in group B and 61 mono-pronucleus(lPN)or tri-pronucleus(3PN)embryoes in group C. The mtDNA copy number in group A (80, 547 + 37, 314 copies/μl) was significantly fewer than that in group B (104,311 + 43,074) copies/μl and group C (192,681 ±51,394) copies/μl(P<0. 01). The mtDNA copy number in group B was significantly fewer than that in group C(P<0. 05). Group C was divided to two subgroup:group Cl (poor quality of embryos and debris more than 5%) and group C2 (normal development of embryos). The mtDNA copy number in group Cl [(146, 836 + 36, 585) copies/μl] was significantly fewer than that in group C2[(297,268 + 102,369) copies/μl](P = 0. 000).Conclusions; Mitochondrial DNA copy number is critical for fertilization outcome and can be as an important marker of oocyte quality. It may explain some cases of fertilization failure.关键词
线粒体/线粒体DNA/卵母细胞/受精/早期胚胎发育Key words
Mitochondrion/mtDNA/Oocyte/Fertilization/Early embryo development引用本文复制引用
戴蜜蜜,胡珍,吕杰强,葛红山..线粒体DNA拷贝数对卵母细胞受精结局的影响[J].生殖医学杂志,2012,21(2):158-161,4.基金项目
国家自然科学基金(30900516) （30900516）
浙江省自然科学基金(Y208100) （Y208100）
