天然产物研究与开发2012，Vol.24Issue(3)：359-361,302,4.
银耳多糖单糖组成分析的三种色谱方法比较
Comparison of Three Chromatographic Methods for Monosaccharide Composition Analysis of Tremella Polysaccharide
摘要
Abstract
To find an accurate and fast method to determine the monosaccharide composition of Tremella polysaccharide, thin layer chromatography ( TLC ) , gas chromatography ( GC) and high performance liquid chromatography ( HPLC ) were compared. The results of TLC and GC were not ideal. However,HPLC was a simple method with high sensitivity, good resolution and integrity information. The result of HPLC analysis showed that the monosaccharide compositions were Glc,Man,GlcA,Xyl and Fuc.with the mole percentage of 0. 24:1.00= 0.06:0. 29: 0. 25. Consequently, HPLC is the most suitable method for moncsaccharide composition analysis.关键词
银耳多糖/单糖组成/TCL/GC/HPLCKey words
Tremella polysaccharide/monosaccharide compositions/TLC/GC/HPLC分类
医药卫生引用本文复制引用
韩威,姜瑞芝,陈英红,高阳,高其品..银耳多糖单糖组成分析的三种色谱方法比较[J].天然产物研究与开发,2012,24(3):359-361,302,4.基金项目
十一五国家科技重大专项(2009ZX09103-333) （2009ZX09103-333）
国家自然科学基金项目(30873370) （30873370）
