天然产物研究与开发2012，Vol.24Issue(3)：393-397,5.
响应面法优化巨大芽孢杆菌中的聚-β-羟基丁酸(PHB)提取工艺
Optimal Extraction Conditions of Poly-β-hydroxybutyrate (PHB) in Bacillus megaterium by Response Surface Methodology
摘要
Abstract
To obtain the optimal extraction conditions of Poly-β-hydroxybutyrate (PHB) in Bacillus megaterium, the effect of chloroform concentration,extraction time and temperature on PHB extraction were studied. On the basis of single factor experiment,Box-Benhnken central composite method was designed for the three factors. In response to PHB extraction value,the extraction conditions were further optimized by response surface method ( RSM). The results snowed that when chloroform concentration was 69.7% .extraction temperature was 59.7 XI .extraction time was 3.0 h.the productivity of PHB was 4.437 g/L, reached its maximum.关键词
巨大芽孢杆菌(Bacillus megaterium)/聚-β-羟基丁酸(PHB)/响应面法(RSM)Key words
Bacillus inegateriuin/poly-β-hydroxybutyrate (PHB) / response surface methodology (B5M)分类
生物科学引用本文复制引用
周琴,董夏梦,汪鹏荣,蓝丽精,嵇豪,蒋冬花..响应面法优化巨大芽孢杆菌中的聚-β-羟基丁酸(PHB)提取工艺[J].天然产物研究与开发,2012,24(3):393-397,5.基金项目
浙江省公益性技术应用研究计划项目(2010C32076) （2010C32076）
浙江省自然科学基金项目(Y3090343) （Y3090343）
浙江省大学生科技创新活动计划(2010R404064) （2010R404064）
