土壤学报2012，Vol.49Issue(2)：347-353,7.
三种农田土壤中氨氧化细菌amoA基因多样性比较分析
COMPARATIVE ANALYSIS OF AMMONIA MONOOXYGENASE (amoA) GENE DIVERSITY IN THREE CULTIVATED SOILS
摘要
Abstract
To analyze by comparison AOB amok gene diversity of three cultivated soils, I. E. Black soil in Hailun (HL) , chao soil in Fengqiu (FQ) and red soil in Yingtan ( YT) which are located in different climatic zones in East China. Total DNA of soil microbe was extracted by the direct lysis method, and amplified with specific primers of AOB amok gene, amok gene libraries of the three soils were thus constructed and analyzed with the RFLP method for diversity. The number of clones in amok gene library was 49, 50 and 48 in HL, FQ and YT respectively, and their corresponding number of OUT (Operational Taxonomic Unit) was 10, 10 and 14, of while 4 were common in all the three samples. YT was the highest in index of amok gene diversity while FQ the lowest; The similarity index between HL and FQ in AOB community was 70% , between HL and YT was 50% , and between FQ and YT was 42% , which indicates that AOB distribution has geographic characteristics. Seventeen AOB amok gene sequences could be sorted into six clusters belonging to two genera, Nitrosospira and Nitrosomonas. All the three soils have an abundance of AOB, thus implying autotroph-ic AOB play a crucial role in nitrogen cycling in cultivated soils.关键词
氨氧化细菌/amoA基因/RFLP分析/农田土壤/微生物多样性Key words
Ammonia-oxidizing bacteria/ Ammonia monooxygenase gene ( amok) / RFLP analysis/ Cultivated soils/ Microbial diversity分类
生物科学引用本文复制引用
汪峰,曲浩丽,丁玉芳,孙波,崔中利,曹慧..三种农田土壤中氨氧化细菌amoA基因多样性比较分析[J].土壤学报,2012,49(2):347-353,7.基金项目
国家自然科学基金项目(40871125,40871123)资助 （40871125,40871123）
