土壤学报2012，Vol.49Issue(2)：354-363,10.
不同干扰方式对喀斯特生态系统土壤细菌优势类群—变形菌群落的影响
EFFECT OF HUMAN DISTURBANCE ON COMPOSITION OF THE DOMINANT BACTERIAL GROUP PROTEOBACTERIA IN KARST SOIL ECOSYSTEMS
摘要
Abstract
With a Karst primeval forest ( KPF) set as check, effects of human disturbance on community structure of soil bacteria were analyzed using the 16S rRNA genes PCR-RFLP and sequencing techniques. Results show that Proteobac-teria were the dominant bacterial group in all the four soils subjected to different patterns of human disturbance, primeval forest (KPF, free of disturbance) , naturally restored land (KNR) , cropland (KMS) and grassland disturbed by long-term grazing and burned annual in winter (KGB) , separately, accounting for 41.3% of the total clone libraries. As compared to KPF, the proportions of Proteobacteria were decreased by 30. 2%-47. 4% in disturbed soils (KNR, KMS and KGB ). Similar distributions of the subgroups of Proteobacteria were found among KPF, KNR and KGB, being in the order of a- > 8- > p- > y-Proteobacteria, but in KMS it was in the order of 8- > a- > p- > y-Proteobacteria, which indicate that the effects of natural restoration and grazing and burning in winter were limited on recovery of soil Proteobacteria, but obviously positive on distribution sequence of the four subphyla. In KNR, the subphylum of a-Proteobacteria was well restored, being 130% higher than in KMS. In the four soils, 16. 5% of the total clones were sorted into Rhizobiales, which was the highest in KPF, about 1. 6 to 3. 7 times higher than in the other three soils. Based on the above-described findings, it is concluded that planting of native nitrogen-fixing plants in combination with inoculation of native nitrogen-fixing microorganisms may be considered as one of the measures in future to restore degraded Karst ecosystems.关键词
喀斯特/人为干扰/16S rRNA/变形菌Key words
Karst/ Human disturbance/ 16S rRNA/ Proteobacteria分类
农业科技引用本文复制引用
陈香碧,苏以荣,何寻阳,覃文更,魏亚伟,梁月明,吴金水..不同干扰方式对喀斯特生态系统土壤细菌优势类群—变形菌群落的影响[J].土壤学报,2012,49(2):354-363,10.基金项目
中国科学院知识创新重要方向项目(KSCX2-YW-436,KZCX2-YW-JC403)、国家自然科学基金面上项目(30970538)和国家科技支撑计划项目(2011BAC09B02,2009BADC6B08)共同资助 （KSCX2-YW-436,KZCX2-YW-JC403）
