外科理论与实践2012，Vol.17Issue(2)：151-156,6.DOI:10.3969/j.issn.1007-1096.2012.02.015
血管紧张素Ⅱ1型受体在人胃癌细胞株中的表达及血管紧张素Ⅱ对MKN-28细胞增殖和周期的影响
Expression of angiotensin Ⅱ type 1 receptor (AT1R) in human gastric cancer cell lines and effects of angiotensin Ⅱ on MKN-28 cell proliferation and cell cycle
范修珂 1黄玮 2冯波 3周厚民 1王蒲雄志 2赵红超 1郑民华 2胡伟国1
作者信息
- 1. 上海交通大学医学院附属瑞金医院外科上海200025
- 2. 上海交通大学医学院附属瑞金医院,上海市微创外科临床医学中心,上海200025
- 3. 上海交通大学医学院附属瑞金医院消化内科,上海200025
- 折叠
摘要
Abstract
Objective To investigate the expression of angiotensin II type 1 receptor (AT1R) in human gastric cancer cell lines, and the effect of angiotensin II (Ang II) on MKN-28 cell proliferation and cell cycle. Methods The AT1R expression in human gastric cancer cell lines was detected by Western blot. The MKN-28 cells were treated with different concentrations of Ang II (10~10 - 10"5 mol/L), AT1R blocker (losartan), and Ang II type 2 receptor(AT2R) blocker (PD123319). Cell proliferation was measured by cell counting kit-8 and cell cycles in different treated groups were measured by flow cytometry. Results Most of the human gastric cancer cell lines expressed AT1R. In these cell lines, MKN-28 cells had the highest level of AT1R expression. Ang H significantly enhanced the MKN-28 cell proliferation and promote the transformation from Gi phase to S, G2 phase in MKN-28 cell line. All of these effects could be inhibited by losartan, but not PD123319. Conclusions Ang II can significantly promote the MKN-28 cell proliferation and accelerate the cell cycle transformation from G[ phase to S, G2 phase via AT1R.关键词
胃癌/血管紧张素Ⅱ/血管紧张素Ⅱ1型受体/细胞增殖Key words
Gastric cancer/ Angiotensin II / Angiotensin II type 1 receptor/ Cell proliferation分类
医药卫生引用本文复制引用
范修珂,黄玮,冯波,周厚民,王蒲雄志,赵红超,郑民华,胡伟国..血管紧张素Ⅱ1型受体在人胃癌细胞株中的表达及血管紧张素Ⅱ对MKN-28细胞增殖和周期的影响[J].外科理论与实践,2012,17(2):151-156,6.
