中国组织工程研究2012，Vol.16Issue(19)：3427-3432,6.DOI:10.3969/j.issn.1673-8225.2012.19.002
骨髓充质干细胞调控尿激酶型纤溶酶原激活物表达及其对大鼠肝星状细胞凋亡的影响
Bone marrow mesenchymal stem cells increased expression of urokinase type plasminogen activator and promoted apoptosis of hepatic stellate cells in rats
摘要
Abstract
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) promote the apoptosis of hepatic stellate cells (HSCs) by paracrine and activation of hepatocyte growth factor (HGF).OBJECTIVE: To investigate the effects of BMSC regulation to urokinase type plasminogen activator on activity of hepatocyte growth factor, and the mechanism by which BMSCs induce the apoptosis of hepatic stellate cells.METHODS: Five groups were divided randomly: Co-culture group, in which rat BMSCs and HSCs were co-cultured by upper and down double-layer co-culture system; HSCs group, in which HSCs were simply cultured; fibroblasts group; UK122 pretreated group: 6 hours before co-culture of BMSCs and HSCs, urokinase type plasminogen activator specific inhibitor UK122 was added; BMSCs blank control group.RESULTS AND CONCLUSION: mRNA expression of urokinase type plasminogen activator in the co-culture group was significantly higher than in the HSCs group (P< 0.01). Compared with HSC group, HSC proliferation was significantly inhibited in the co-culture group at 24 hours (P < 0.01) in a time-dependent manner. After co-culture, HGF protein expression and HSC apoptosis were significantly increased (P < 0.01). After UK122 intervention, HGF expression and HSC apoptosis were significantly decreased (P < 0.01). These findings suggest that BMSCs promote the secretion of urokinase type plasminogen activator, increase the expression of active HGF, and promote the apoptosis of HSCs.关键词
肝星状细胞/骨髓间充质干细胞/凋亡/肝细胞生长因子/尿激酶型纤溶酶原激活物分类
医药卫生引用本文复制引用
宁琳,姜海行,覃山羽,张君红,杨文,孟云超..骨髓充质干细胞调控尿激酶型纤溶酶原激活物表达及其对大鼠肝星状细胞凋亡的影响[J].中国组织工程研究,2012,16(19):3427-3432,6.基金项目
广西自然科学基金资助项目(0897008),广西"新世纪十百千人才工程"专项基金资助项目(2006206). （0897008）
