中国组织工程研究2012，Vol.16Issue(19)：3438-3441,4.DOI:10.3969/j.issn.1673-8225.2012.19.004
Transwell小室环境下兔成骨样细胞与骨髓基质干细胞的共培养及成骨分化
Co-cultivation of bone marrow stromal cells with rabbit osteoblast-like cells in a Transwell chamber for osteogenic differentiation
张树鹰 1段大波 2张力 2王稚英1
作者信息
- 1. 辽宁医学院附属第二医院,辽宁省锦州市,121000
- 2. 锦州市中心医院,辽宁省锦州市,121000
- 折叠
摘要
Abstract
BACKGROUND: Osteogenic differentiation is previously limited by culture of dexamethasone, growth factors or osteoblast-like cells with bone marrow mesenchymal stem cells at 1:1.OBJECTIVE: To investigate the feasibility of in vitro co-cultivation of osteoblast-ike cells and bone marrow stromal cells (BMSCs) and osteoblast differentiation of BMSCs by osteoblast-like cells.METHODS: Passage 3 rabbit osteoblast-like cells and passage 3 rabbit BMSCs were co-cultured in Transwell chamber, and osteoblast-like cells were inoculated into the lower part of culture plate. BMSCs were inoculated on Transwell inner membrane, serving as experimental group. BMSCs were inoculated into Transwell chamber alone with basal medium in the lower part, serving as control group.RESULTS AND CONCLUSION: In the experimental group, BMSCs differentiated toward osteoblasts, and alkaline phosphatase activity was significantly higher compared with the control group (P < 0.05). In the experimental group, BMSCs were positive for alizarin red staining, showing red calcified nodules; after RT-PCR, the expression of core-binding factor α1 was observed. In the control group, calcified nodules were not observed. These findings suggest that Transwell chamber can help to achieve the in vitro co-cultivation of osteoblast-like cells and BMSCs and the osteogenic differentiation of BMSCs.关键词
成骨样细胞/Transwell小室/骨髓基质干细胞/共培养/成骨分化/兔分类
医药卫生引用本文复制引用
张树鹰,段大波,张力,王稚英..Transwell小室环境下兔成骨样细胞与骨髓基质干细胞的共培养及成骨分化[J].中国组织工程研究,2012,16(19):3438-3441,4.
