中国组织工程研究2012，Vol.16Issue(19)：3447-3452,6.DOI:10.3969/j.issn.1673-8225.2012.19.006
血管内皮细胞对联合培养体系中骨髓间充质干细胞侏儒症相关转录因子表达及成骨分化的影响
Effects of vascular endothelial cells on runt-related transcription factor 2 expression and osteogenic differentiation of bone marrow mesenchymal stem cells in a co-cultivation system
摘要
Abstract
BACKGROUND: Vascular endothelial cells can promote osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), which provide nutrition supports for osteogenic differentiation of stem cells.OBJECTIVE: To investigate the effects of human umbilical vein endothelial cells (HUVECs) on the morphology, growth, differentiation of BMSCs as well as the runt-related transcription factor 2 (Runx2) gene expression in a co-cultivation system. METHODS: In a co-cultivation system, passage 3 BMSCs and passage 3 HUVECs were cultured together and served as experimental group. Passage 3 BMSCs were simply cultured and served as control group. At 4, 6, 10 days of culture, BMSCs morphology, alkaline phosphatase activity and Runx2 gene expression were determined.RESULTS AND CONCLUSION: Compared with control group, BMSCs morphology was diverse, and cell conjunction and fusion appeared, and osteogenic differentiation was obvious during anaphase in the experimental group. At each time point, there was no obvious change in alkaline phosphatase activity in the control group, and alkaline phosphatase activity was significantly higher in the experimental group than in the control group (P < 0.05). At 6 and 8 days, Runx2 gene expression in the experimental group was about 4 times that in the control group (P < 0.01). This suggests that in the co-cultivation system, intravenous endothelial cells can promote Runx2 expression in BMSCs and induce osteogenic differentiation of BMSCs.关键词
血管内皮细胞/骨髓间充质干细胞/侏儒相关转录因子(Runx-2)/联合培养/实时定量PCR分类
医药卫生引用本文复制引用
杨瑞年,刘流,王福科,赵德萍..血管内皮细胞对联合培养体系中骨髓间充质干细胞侏儒症相关转录因子表达及成骨分化的影响[J].中国组织工程研究,2012,16(19):3447-3452,6.基金项目
国家自然科学基金资助项目(30960388 （）
81160239). （）
