中国组织工程研究2012，Vol.16Issue(19)：3492-3495,4.DOI:10.3969/j.issn.1673-8225.2012.19.015
脂肪组织来源干细胞可促进脂肪移植物的存活率
Adipose tissue derived stem cells increase the survival rate of fat grafts
杨波 1沈宏亮 1徐志飞 1李嵩 1陈坤1
作者信息
- 1. 解放军第二军医大学第二附属医院,上海市,200003
- 折叠
摘要
Abstract
BACKGROUND: The adipose tissue derived stem cells (ADSCs) can secrete cell factors to promote neovascularization following directional induction in vitro.OBJECTIVE: To estimate the effects of ADSCs on the survival rate of fat grafts following the mixed transplantation of ADSCs and free fat tissues.METHODS: ADSCs were transfected by recombinant adenovirus encoding green fluorescent protein (Ad-EGFP) gene with different multiplicity of infection (MOI) values. Ad-EGFP-transfected cells at the ideal MOI were divided into experimental group and control group. Mixed transplantation of ADSCs and fat tissues into the back of nude mice was performed in the experimental group, and the simple fat tissues transplantation was taken in the control group.RESULTS AND CONCLUSION: Green fluorescence could be seen in ADSCs at 24 hours after transfection, the expression intensity at 5 days was the strongest, and the EGFP was still expressed after passage. The ideal transfection rate was 95.3% at a MOI value of 50. The ADSCs in the experimental group scattered in the fat cells and interlobular septum. Some of the ADSCs could be differentiated into vascular endothelial cells. The wet mass and survival rate of fat grafts in the experimental group were improved compared with the control group (P = 0.001), but the fat graft fibrogenesis and damaged condition were decreased (P = 0.001). The transplantation of ADSCs can increase the survival rate of fat grafts through promoting the angiopoiesis of free fat tissues.关键词
脂肪组织来源干细胞/脂肪组织/血管形成/绿色荧光蛋白/干细胞移植分类
医药卫生引用本文复制引用
杨波,沈宏亮,徐志飞,李嵩,陈坤..脂肪组织来源干细胞可促进脂肪移植物的存活率[J].中国组织工程研究,2012,16(19):3492-3495,4.
