中国组织工程研究2012，Vol.16Issue(19)：3551-3555,5.DOI:10.3969/j.issn.1673-8225.2012.19.027
RNA干扰抑制骨髓间充质干细胞中caspase-3基因的表达
RNA interference inhibits caspase-3 gene expression in bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND: Poor viability of bone mesenchymal stem cells after transplantation into the infarcted region has limited the reparative capacity of these cells because of the apoptosis.OBJECTIVE: To construct eukaryotic vector expressing small interfering RNAs against caspase-3 and investigate the inhibitory effect on the expression of caspase-3 in BMSCs in vitro.METHODS: According to the principle of siRNA design, siRNAs against caspase-3 gene were designed and synthesized. Eukaryotic vector expressing caspase-3siRNA was constructed and transferred into BMSCs with the liposome conduct. There were 5 groups, including normal cell group, negative sequence group and 3 interfering groups (si-caspase-3-1, si-caspase-3-2 and si-caspase-3-3).RESULTS AND CONCLUSION: Eukaryotic vector expressing caspase-3siRNA was constructed and transferred into BMSCs successfully. Western blot analysis and reverse-transcription polymerase chain reaction demonstrated that levels of of caspase-3 protein and mRNA in transfected BMSCs decreased, with the most significant decline in the group with si-caspase-3-3. Eukaryotic vector expressing caspase-3 siRNA could inhibit the expression of caspase-3 gene in BMSCs in vitro.关键词
骨髓间充质干细胞/凋亡/caspase-3/RNA干扰/抑制效应分类
医药卫生引用本文复制引用
刘立宝,华平,杨淞然,刘家良,江慧琦,王萌,曾宽,敖翔,杨艳旗..RNA干扰抑制骨髓间充质干细胞中caspase-3基因的表达[J].中国组织工程研究,2012,16(19):3551-3555,5.基金项目
广东省自然科学基金资助项目(7001619) （7001619）
广东省科技计划项目(2007B050200011). （2007B050200011）
